Principal Investigators: Bernadette Julier

Associated with: INRA

Project Summary:

EUCLEG aims to reduce Europe and China’s dependency on protein imports by developing efficient breeding strategies for the legume crops of major economic importance in human food and animal feed. The objective is to improve diversification, crop productivity, yield stability and protein quality of both forage (alfalfa and red clover) and grain (pea, faba bean and soybean) legumes. Using diverse and extensive genetic resources and taking advantage of advanced molecular tools, EUCLEG aims to identify and develop the best genetic resources, phenotyping methods and molecular tools to breed legume varieties with improved performance under biotic and abiotic stresses in the representative European and Chinese agro-ecological areas. The potential for new uses of forage species for human nutrition will be explored. Searchable databases will be developed or built to host passport, agronomic and genetic data facilitating exchanges and use of genetic resources. The evaluation of genetic resources in multi-site trials will allow to broaden the breeding material and extend agro-ecological adaptation. The genetic architecture of key breeding traits will be analysed using association studies in order to identify molecular markers related to phenotypic traits. Finally, genomic selection strategies will be assessed for their potential to improve genetic progress. Practical tools for genotyping, data management and calculation will be provided to breeders to implement marker-assisted selection and genomic selection leading to the creation of new varieties in the long term. The partnership gathered in EUCLEG, combining public institutes and private companies of Europe and China,
guaranties the transfer of knowledge from research to seed industry.

Link(s): http://www.eucleg.eu

Name:

Guohua Xu

Job Title:

Professor

Email:

ghxu@njau.edu.cn

Phone Number:

0086-25-84396246

Links:

Google Scholar Profile

Biography:

I am a Professor at Nanjing Agricultural University. My work focuses on the physiology and molecular genetics of plant adaption to altered availability of major nutrients and salt stress.

Research Areas:

• Plant mineral nutrition

Current N-related Projects:

• Improving crop N use efficiency

Bibliography:

1. Shunan Zhang, Yuyi Zhang, Kangning Li, Ming Yan, Jinfei Zhang, Ming Yu, Shuo Tang, Luyang Wang, Hongye Qu, Le Luo, Wei Xuan, Guohua Xu*. 2020. Nitrogen mediates flowering time and nitrogen use efficiency via a floral regulator in rice. Current Biology DOI: 10.1016/j.cub.2020.10.095.

2. Mengmeng Hou, Feifei Luo, Daxia Wu, Xuhong Zhang, Manman Lou, Defeng Shen, Ming Yan, Chuanzao Mao, Xiaorong Fan, Guohua Xu, Yali Zhang*. 2020. OsPIN9, an auxin efflux carrier, is required for the regulation of rice tiller bud outgrowth by ammonium. New Phytologist DOI: 10.1111/nph.16901.

3. Shang Pan, Yang Wang, Yunpeng Qiu, Dima Chen, Lin Zhang, Chenglong Ye, Hui Guo, Weixing Zhu, Aiqun Chen, Guohua Xu, Yi Zhang*, Yongfei Bai*, Shuijin Hu*. 2020. Nitrogen‐induced acidification, not N‐nutrient, dominates suppressive N effects on arbuscular mycorrhizal fungi. Global Change Biology. https://doi.org/10.1111/gcb.15311

4. Guohua Xu*, Hideki Takahashi*. 2020. Improving nitrogen use efficiency: from cells to plant systems. Journal of Experimental Botany 71 (15), 4359-4364.

5. Shuangshuang Wang, Aiqun Chen*, Kun Xie, Xiaofeng Yang, Zhenzhen Luo, Jiadong Chen, Dechao Zeng, Yuhan Ren, Congfan Yang, Lingxiao Wang, Huimin Feng, Damar Lizbeth Lopez-Arredondo, Luis Rafael Herrera-Estrella*, Guohua Xu * (徐国华). 2020. Functional analysis of the OsNPF4.5 nitrate transporter reveals a conserved mycorrhizal pathway of nitrogen acquisition in plants. Proceedings of the National Academy of Sciences of the United States of America. www.pnas.org/cgi/doi/10.1073/pnas.2000926117.

6. Chen JG, Liu XQ, Liu SH, Fan XR, Zhao L, Song MQ, Fan XR*, Xu GH. 2020. Co-Overexpression of OsNAR2. 1 and OsNRT2. 3a Increased Agronomic Nitrogen Use Efficiency in Transgenic Rice Plants. Frontiers in Plant Science 11, 1245.

7. Guo N, Gu MJ, Hu J, Qu HY*, Xu G. 2020. Rice OsLHT1 Functions in Leaf-to-Panicle Nitrogen Allocation for Grain Yield and Quality. Frontiers in Plant Science 11, 1150.

8. Nan Guo, Jinqi Hu, Ming Yan, Le Luo, Hongye Qu, Mechthild Tegeder*, Guohua Xu*. 2020. Oryza sativa Lysine-Histidine-type Transporter 1 functions in root uptake and root-to-shoot allocation of amino acids in rice. The Plant Journal 103(1): 395-411. https://doi.org/10.1111/tpj.14742.

9. Miaoquan Song, Xiaorong Fan, Jingguan Chen, Hongye Qu, Le Luo, Guohua Xu*. 2020. Characterization of OsNAR2.1 interaction with OsNIT1 and OsNIT2 in rice uncovers their different critical roles in mediating root growth response to nitrate and ammonium. Plant Physiology DOI:10.1104/pp.19.01364.

10. Le Luo, Yali Zhang, Guohu Xu*. 2020. How does nitrogen shape plant architecture? Journal of Experimental Botany https://doi.org/10.1093/jxb/eraa187

11. Huimin Feng, Xiaorong Fan, Anthony J Miller, Guohua Xu*. 2020. Plant nitrogen uptake and assimilation: regulation of cellular pH homeostasis. Journal of Experimental Botany https://doi.org/10.1093/jxb/eraa150.

12. Zhizhong Gong†, Liming Xiong†, Huazhong Shi†, Shuhua Yang†, Luis R. Herrera-Estrella†, Guohua Xu†, Dai-Yin Chao†, Jingrui Li, Peng-Yun Wang, Feng Qin, Jijang Li, Yanglin Ding, Yiting Shi, Yu Wang, Yongqing Yang, Yan Guo* and Jian-Kang Zhu*. 2020. Plant abiotic stress response and nutrient use efficiency. SCIENCE CHINA Life Sciences. 63, doi: 10.1007/s11427-020-1683-x († equal contribution).

13. Fan X, Chen J, Wu Y, Teo CH, Xu G, Fan X*. 2020. Genetic and Global Epigenetic Modification, Which Determines the Phenotype of Transgenic Rice? International Journal of Molecular Sciences 21 (5), 1819.

14. Luo B, Xu M, Zhao L, Xie P, Chen Y, Harwood W, Xu G, Fan X*, Miller AJ*. 2020. Overexpression of the High-Affinity Nitrate Transporter OsNRT2. 3b Driven by Different Promoters in Barley Improves Yield and Nutrient Uptake Balance. International Journal of Molecular Sciences 21 (4), 1320.

15. Junli Liu, Jiadong Chen, Kun Xie, Yuan Tian, Anning Yan, Jianjian Liu, Yujuan Huang, Shuangshuang Wang, Yiyong Zhu, Aiqun Chen*, Guohua Xu*. 2020. A mycorrhiza‐specific H+‐ATPase is essential for arbuscule development and symbiotic phosphate and nitrogen uptake. Plant Cell and Environment https://doi.org/10.1111/pce.13714.

16. Gao Z, Wang Y, Chen G, Zhang A, Yang S, Shang L, Wang D, Ruan B, Liu C, Jiang H, Dong G, Zhu L, Hu J, Zhang G, Zeng D, Guo L, Xu GH, Teng S, Harberd NP*, Qian Q*. 2019. The indica nitrate reductase gene OsNR2 allele enhances rice yield potential and nitrogen use efficiency. Nature Communication 10(1):5207.

17. Dong C, Li F, Yang T, Feng L, Zhang S, Li F, Li W, Xu G, Bao S, Wan X, Lucas WJ, Zhang Z. 2019. Theanine transporters identified in tea plants (Camellia sinensis L.). The Plant Journal. 101 (1), 57-70.

18. Huang S, Liang Z, Chen S, Sun H, Fan X, Wang C, Xu GH, Zhang YL*. 2019. A Transcription Factor, OsMADS57, Regulates Long-Distance Nitrate Transport and Root Elongation. Plant Physiology. 180(2):882-895.

19. Chen J, Qi T, Hu Z, Fan X, Zhu L, Iqbal MF, Yin X, Xu G, Fan X*. 2019. OsNAR2.1 Positively Regulates Drought Tolerance and Grain Yield Under Drought Stress Conditions in Rice. Frontiers in Plant Science. 10:197. doi: 10.3389/fpls.2019.00197.

20. Wei J, Zheng Y, Feng HM, Qu HY, Fan XR, Yamaji N, Ma JF, Xu GH*. 2018. Rice OsNRT2.4 gene encodes a dual-affinity nitrate transporter and functions in nitrate-regulated root growth and nitrate distribution. Journal of Experimental Botany 69: 1095-1107.

21. Luo L, Qin R, Liu T, Yu M, Yang T, Xu G*. 2018. OsASN1 Plays a Critical Role in Asparagine-Dependent Rice Development. International Journal of Molecular Sciences 20(1). doi: 10.3390/ijms20010130.

22. Pei W, Jain A, Ai H, Liu X, Feng B, Wang X, Sun Y, Xu G, Sun S*. 2018. OsSIZ2 regulates nitrogen homeostasis and some of the reproductive traits in rice. Journal of Plant Physiology 232: 51-60.

23. Sun Y, Luo W, Jain A, Liu L, Ai H, Liu X, Feng B, Zhang L, Zhang Z, Xu G, Sun S*. 2018. OsPHR3 affects the traits governing nitrogen homeostasis in rice. BMC Plant Biology. 18(1):241. doi: 10.1186/s12870-018-1462-7.

24. Luo B, Chen J, Zhu L, Liu S, Li B, Lu H, Ye G, Xu G, Fan X*. 2018. Overexpression of a High-Affinity Nitrate Transporter OsNRT2.1 Increases Yield and Manganese Accumulation in Rice Under Alternating Wet and Dry Condition. Frontiers in Plant Science 9:1192. doi: 10.3389/fpls.2018.01192.

25. Sun H, Tao J, Bi Y, Hou M, Lou J, Chen X, Zhang X, Luo L, Xie X, Yoneyama K, Zhao Q, Xu G, Zhang Y*. 2018. OsPIN1b is Involved in Rice Seminal Root Elongation by Regulating Root Apical Meristem Activity in Response to Low Nitrogen and Phosphate. Sci Rep. 8(1):13014.

26. Luo L, Wang HX, Liu XH, Hu JQ, Zhu XL, Pan S, Qin RY, Wan YF, Zhao PP, Fan XR, Xu GH*. 2018. Strigolactones affect the translocation of nitrogen in rice. Plant Science. 270: 190-197.

27. Guohua Xu*. 2018. Sensing and transport of nutrients in plants. Seminars in Cell & Developmental Biology 74: 78-79.

28. Chen AQ, Gu M, Wang SS, Chen J, Xu GH*. 2018. Transport properties and regulatory roles of nitrogen in arbuscular mycorrhizal symbiosis. Seminars in Cell & Developmental Biology 74: 80-88.

29. Xuan W, Beeckman T, Xu GH*. 2017. Plant nitrogen nutrition: sensing and signaling. Current Opinion in Plant Biology 39: 57-65.

30. Feng HM, Li B, Zhi Y, Chen J, Li R, Xia XD, Xu GH, Fan XR*. 2017. Overexpression of the nitrate transporter, OsNRT2.3b, improves rice phosphorus uptake and translocation. Plant Cell Reports 36: 1287-1296.

31. Guo H, Zhou H, Zhang J, Guan W, Xu S, Shen W, Xu GH, Xie Y*, Foyer C. 2017. L-cysteine desulfhydrase-related H2S production is involved in OsSE5-promoted ammonium tolerance in roots of Oryza sativa. Plant Cell & Environment 40: 1777-1790.

32. Chen J, Fan XR, Qian K, Zhang Y, Song M, Liu Y, Xu GH, Fan XR*. 2017. pOsNAR2.1:OsNAR2.1 expression enhances nitrogen uptake efficiency and grain yield in transgenic rice plants. Plant Biotechnology Journal 15: 1273-1283.

33. Fan XR; Naz M; Fan XR; Xuan W; Miller AJ; Xu GH* 2017. Plant nitrate transporters: from gene function to application. Journal of Experimental Botany 68(10): 2463–2475.

34. Sun, Huwei; Tao, Jinyuan; Zhao, Quanzhi; Xu, Guohua; Zhang, Yali. 2017. Multiple roles of nitric oxide in root development and nitrogen uptake. Plant Signaling & Behavior 12(1): e1274480．

35. Gao CM, Ding L, Li YR, Chen YP, Zhu JW, Gu M, Li Y, Xu GH, Shen QR, Guo SW. 2017. Nitrate increases ethylene production and aerenchyma formation in roots of lowland rice plants under water stress. Functional Plant Biology 44: 430-442.

36. Luo L; Pan S; Liu XH; Wang HX; Xu GH*. 2017. Nitrogen deficiency inhibits cell division-determined elongation, but not initiation, of rice tiller buds. Israeli Journal of Plant Science 64: 32-40.

37. Li C, Tang Z, Wei J, Qu HY, Xie YJ, Xu GH*. 2016. The OsAMT1.1 gene functions in ammonium uptake and ammonium-potassium homeostasis over low and high ammonium concentration ranges. Journal of Genetics and Genomics 43(11): 639-649.

38. Fan XR, Tang Z, Tan YW, Zhang Y, Luo BB, Yang M, Lian XM, Shen QR, Miller AJ, Xu GH*. 2016. Overexpression of a pH-sensitive nitrate transporter in rice increases crop yields. Proceedings of the National Academy of Sciences of the United States of America. 113: 7118-7123.

39. Chen JG, Zhang Y, Tan YW, Zhang M, Zhu LL, Xu GH, Fan XR. 2016. Agronomic nitrogen-use efficiency of rice can be increased by driving OsNRT2.1 expression with the OsNAR2.1 promoter. Plant Biotechnology Journal 14(8):1705-1715.

40. Sun HW, Bi Y, Tao JY, Huang SJ, Hou MM, Xue R, Ling ZH, Gu PY, Yoneyama K, Xie XN, Shen QR, XU GH, Zhang YL. 2016. Strigolactones are required for nitric oxide to induce root elongation in response to nitrogen- and phosphate-deficiency in rice. Plant Cell & Environment 39: 1473-84.

41. Wang HD, Sun R, Cao Y, Pei WX, Sun YF, Zhou HM, Wu XN, Zhang F, Luo L, Shen QR, Xu GH, Sun SB. 2015. OsSIZ1, a SUMO E3 Ligase Gene, is Involved in the Regulation of the Responses to Phosphate and Nitrogen in Rice. Plant & Cell Physiology 56(12):2381-95.

42. Zhu J, Liang J, Xu Z, Fan X, Zhou Q, Shen Q, Xu G. 2015. Root aeration improves growth and N accumulation in rice seedlings under low nitrogen. AoB Plants. 7: doi:10.1093/aobpla/plv131.

43. Huang SJ, Chen S, Liang Z, Zhang CM, Yan M, Chen JG, XU GH, Fan X, Zhang YL. 2015. Knockdown of the partner protein OsNAR2.1 for high-affinity nitrate transport represses lateral root formation in a nitrate-dependent manner. Scientific Reports. 5:18192. doi: 10.1038/srep18192.

44. Lei Ding, Limin Gao, Wei Liu, Min Wang, Mian Gu, Binbin Ren, Guohua Xu, Qirong Shen, Shiwei Guo. 2015. Aquaporin plays an important role in mediating chloroplastic CO2 concentration under high-N supply in rice (Oryza sativa) plants. Physiologia Plantarum 156: 215-226.

45. Xiaorong Fan, Huimin Feng, Yawen Tan, Yanling Xu, Qisong Miao, Guohua Xu. 2015. A putative 6 trans-membrane nitrate transporter OsNRT1.1b plays a key role in rice under low nitrogen. Journal of Integrative Plant Biology. 58(6): 590-599.

46. Xiaoqin Liu, Huimin Feng, Daimin Huang, Miaoquan Song, Xiaorong Fan, Guohua Xu*. 2015. Two short sequences in OsNAR2.1 promoter are necessary for fully activating the nitrate induced gene expression in rice roots. Scientific Reports. 5:11950 | DOI: 10.1038/srep11950.

47. Huwei Sun, Jiao Li, Wenjing Song, Jinyuan Tao, Shuangjie Huang, Si Chen, Mengmeng Hou, Guohua Xu and Yali Zhang. 2015. Nitric oxide generated by nitrate reductase increases nitrogen uptake capacity by inducing lateral root formation and inorganic nitrogen uptake under partial nitrate nutrition in rice. Journal of Experimental Botany 66(9): 2449-2459.

48. Ding L., Gao C., Li Y., Li Y., Zhu Y., Xu G., Shen Q., Kaldenhoff R., Kai L. & Guo S. (2015) The enhanced drought tolerance of rice plants under ammonium is related to aquaporin (AQP). Plant Science 234, 14-21.

49. Xiudong Xia, Xiaorong Fan, Jia Wei, Huimin Feng, Hongye Qu, Dan Xie, Anthony J Miller, Guohua Xu* (2014) Rice Nitrate Transporter OsNPF2.4 Functions in Low Affinity Acquisition and Long-Distance Transport. Journal of Experimental Botany 66 (1), 317-331.

50. Liu X, Huang D, Tao J, Miller AJ, Fan X, Xu G. (2014) Identification and functional assay of the interaction motifs in the partner protein OsNAR2.1 of the two-component system for high-affinity nitrate transport. New Phytologist 204(1):74-80.

51. Xie YJ, Mao Y, Xu S, Zhou H, Duan XL, Cui W, Zhang J, Xu GH*. (2014) Heme-heme oxygenase1 system is involved in ammonium tolerance by regulating antioxidant defense in Oryza sativa. Plant Cell and Environment 38 (1), 129-143.

52. Xiaorong Fan, Dan Xie, Jingguang Chen, Haiyan Lu, Yanling Xu, Cui Ma, Guohua Xu. (2014) Over-expression of OsPTR6 in rice increased plant growth at different nitrogen supplies but decreased nitrogen use efficiency at high ammonium supply. Plant Science 384 (1-2), 259-270.

53. Wang M, Shen Q, Xu GH, Guo S. 2014. New insight into the strategy for nitrogen metabolism in plant cells. International Review of Cell and Molecular Biology. 310:1-37.

54. Sun HW, Tao JY, Liu SJ, Huang SJ, Chen S, Xie XN, Yoneyama K, Zhang YL, XU GH (2014) Strigolactones are involved in phosphate and nitrate deficiency-induced root development and auxin transport in rice. Journal of Experimental Botany 65 (22), 6735-6746.

55. X Zhan, J Yuan, L Yue, G Xu, B Hu, R Xu. (2014) Response of uptake and translocation of phenanthrene to nitrogen form in lettuce and wheat seedlings. Environ Sci Pollut Res. 22:6280–6287.

56. Feng HM, Xia XD, Fan XR, Xu GH, Miller AJ. (2013) Optimizing plant transporter expression in Xenopus oocytes. Plant Methods 9:48.

57. Song WJ, Li J, Sun HW, Huang SJ, Gong XP, Ma QY, Zhang YL, Xu GH. 2013. Increased photosynthetic capacity in response to nitrate is correlated with enhanced cytokinin levels in rice cultivar with high responsiveness to nitrogen nutrients. Plant and Soil 373: 981-993.

58. Song W, Sun H, Li J, Gong X, Huang S, Zhu X, Zhang Y, Xu G. (2013) Auxin distribution is differentially affected by nitrate in roots of two rice cultivars differing in responsiveness to nitrogen. Annals of Botany. 112(7): 1383-93.

59. Yong Li, Binbin Ren, Xiuxia Yang, Guohua Xu, Qirong Shen and Shiwei Guo. (2012) Chloroplast Downsizing Under Nitrate Nutrition Restrained Mesophyll Conductance and Photosynthesis in Rice (Oryza sativa L.) Under Drought Conditions. Plant and Cell Physiology 53: 892-900.

60. Zhong Tang, Xiaorong Fan, Qing Li, Huimin Feng, Anthony J. Miller, Qirong Shen, Guohua Xu* (2012) Knock Down of a Rice Stelar Nitrate Transporter Alters Long Distance Translocation but not Root Influx. Plant Physiology 160:2052-2063.

61. Zeng HQ, Liu G, Kinoshita T, Zhang RP, Zhu YY, Shen QR, Xu GH. 2012. Stimulation of phosphorus uptake by ammonium nutrition involves plasma membrane H+ ATPase in rice roots. Plant and Soil 357: 205-214.

62. Xu GH*, Fan XR, Miller AJ. 2012. Plant Nitrogen Assimilation and Use Efficiency. Annual Review of Plant Biology 63: 153-182.

63. Wu P, Xu GH, Lian X. 2012. Nitrogen and phosphorus uptake and utilization. Genetics and Genomics of Rice. Plant Genetics and Genomics: Crops and Models. 5: 217-226.

64. Liu, Anthony J. Miller and Guohua Xu*. 2011. Multiple roles of nitrate transport accessory protein NAR2 in plants. Plant Signaling & Behavior 6(9):1-4.

65. Yan M, Fan XR, Feng HM, Miller AJ, Shen QR, Xu GH*. 2011. Rice OsNAR2.1 interacts with OsNRT2.1, OsNRT2.2 and OsNRT2.3a nitrate transporters to provide uptake over high and low concentration ranges. Plant Cell and Environment. 34:1360-1372.

66. Huimin Feng, Ming Yan, Xiaorong Fan, Baozhen Li, Qirong Shen, Anthony J Miller, Guohua Xu*. 2011. Spatial expression and regulation of rice high-affinity nitrate

transporters by nitrogen and carbon status. Journal of Experimental Botany 62 (7):2319-2332.

67. Song WJ, Makeen K, Wang DS, Zhang CM, Xu YH, Zhao H, Tu E, Zhang YL, Shen QR, Xu GH. 2011. Nitrate supply affects root growth differentially in two rice cultivars differing in nitrogen use efficiency. Plant and Soil 343(1&2):357-368.

68. Miller AJ, Shen QR, Xu GH. 2009. Freeways in the plant: transporters for N, P and S and their regulation. Current Opinion in Plant Biology 12: 284-290.

69. Zhu Y, DI T, Xu G, Chen X, Zeng H, Yan F, Shen Q. 2009. Adaptation of plasma membrane H+-ATPase of rice roots to low pH as related to ammonium nutrition. Plant Cell and Environment. 32(10): 1428-1440.

70. Yuefeng Li, Wei Ran, Ruiping Zhang, Guohua Xu*. 2009. Facilitated mycorrhizal colonization, legume nodulation, phosphate uptake and nitrogen transfer in a mycorrhizal upland rice and mungbean by intercrop. Plant and Soil 315: 285-296.

71. Y. Duan, Y. Zhang, L. Ye, X. Fan, G. Xu, Q. Shen 2007. Responses of Rice Cultivars with Different Nitrogen Use Efficiency to Partial Nitrate Nutrition. Annals of Botany 99(6): 1153-1160.

72. Baozhen Li, Weijie Xin, Shubin Sun, Guohua Xu*. 2006. Physiological and Molecular Responses of Nitrogen-Starved Rice Plants to Re-supply of Different Nitrogen Sources. Plant and Soil. 287:145-159.

73. Yan X, Wu P, Ling H, Xu G, Xu F, Zhang Q. 2006. Plant Nutriomics in China: An Overview. Annals of Botany 98: 473-482.

74. Xiaoqin Qian, Qirong Shen, Guohua Xu*. Juanjuan Wang and Mingyao Zhou. 2004. Nitrogen form effects on yield and nitrogen uptake of rice crop grown in aerobic soil. Journal of Plant Nutrition 27: 1061-1076.

75. Guohua Xu* and Uzi Kafkafi. 2003. Seasonal differences in mineral contents, distribution and leakage of sweet pepper seeds. Annals of Applied Biology 143 (1): 45-52.

76. Guohua Xu*, Uzi Kafkafi, Shmuel Wolf, and Yukihiro Sugimoto. 2002. Mother plant nutrition and growing condition affect amino and fatty acids composition of hybrid sweet pepper seeds. Journal of Plant Nutrition 25 (7): 1645 – 1665.

77. Guohua Xu*, Shmuel Wolf, and Uzi Kafkafi. 2002. Ammonium on Potassium Interaction in Sweet Pepper. Journal of Plant Nutrition 25: 719-734.

78. Guohua Xu, Shmuel. Wolf, and Uzi Kafkafi. 2001. Interaction of nutrient concentration and container volume on flowering, fruiting, and nutrient uptake of sweet pepper. Journal of Plant Nutrition 24: 479-501.

79. Guohua Xu* and Uzi Kafkafi. 2001. Nutrient supply and container size effects on flowering, fruiting assimilate allocation, and water relations of sweet pepper. Acta Horticulture 554: 113-120.

80. Guohua Xu*, Shmuel Wolf, and Uzi Kafkafi. 2001. Effect of varying nitrogen form and concentration during growing season on sweet pepper flowering and fruit yield. Journal of Plant Nutrition 24: 1099-1116.

Name:

Craig Wood

Job Title:

Principle Investigator

Email:

craig.wood@csiro.au

Phone Number:

435400113

Links:

Google Scholar Profile

Biography:

Craig is plant metabolic engineer spanning gene discovery, multistep pathway assemblies and deployment of GM crops into the field. Craig has developed world leading capabilities in plant synthetic biology including high throughput metabolic pathway engineering in plants in the model plant Nicotiana benthamiana, and the crops of safflower and canola. Craig has published in plant sciences in areas such as nitrogen fixation, ammonia transport processes, flowering time control, genome sequencing, plant tissue techniques and in the use of combinatorial metabolic engineering to rapidly assemble new pathways in oil synthesis and nitrogenase engineering.

Craig studied agriculture at high school, undergraduate and postgraduate degrees (University of Sydney). He has postdoctoral experience in Oxford and Berlin before moving to CSIRO situated in Canberra.

Craig has supervised 2 PhD students, Dr Fatima Naim (Using viral suppressor proteins to improve performance of transgenic traits) and Dr Darren Cullerne (Genetics of vernalisation in safflower).

Craig is currently leading a team on engineering bacterial nitrogenase directly in plant cells. Craig is always looking for talented new team members

Research Areas:

• Synthetic biology of plants

Current N-related Projects:

• Transferring nitrogen fixation pathways directly into plants

Bibliography:

See Google Scholar Profile

Name:

Professor Wim de Vries

Job Title:

Professor Integrated nutrient impact modelling

Email:

wim.devries@wur.nl

Phone Number:

+31 317-486514

Links:

Google Scholar Profile

Biography:

Prof. Dr. Ir. Wim de Vries is a principal research scientist at Wageningen University and Research in the field of soil chemistry with special reference to soil acidification, nutrient cycling, greenhouse gas emissions and heavy metal pollution. He is also professor at the Environmental Systems Analysis Group of Wageningen University where he holds the chair “Integrated nutrient impact modelling”. His research is organized around impacts of the elevated use of nutrients in agriculture on soil and water quality, greenhouse gas emissions, productivity and plant species diversity of terrestrial ecosystems and related critical loads.
He is component leader of a global scale GEF/UNEP funded Integrated project entitled “Towards an International Nitrogen Management System” (‘Towards INMS’) with 72 participating institutes (2016-present).

Research Areas:

• Modelling nitrogen and phosphorous cycling through agricultural systems
• Ammonia and greenhouse gas emissions from agriculture
• Impacts of nitrogen inputs on soil acidification
• Impacts of nutrient deposition on carbon sequestration, plant species diversity

Current N-related Projects:

• Fate of nitrogen, phosphorus and metals and impacts on food, soil and water quality (EEA)
• Assessment of spatially explicit needed and critical input levels of nitrogen in agriculture within EU-27 (Fertilizers Europe)
• Global & regional quantification of N use, flows, impacts & benefits of practices (GEF, towards INMS)
• Options for closing mineral imbalances in carbon, nitrogen and phosphorus in Dutch agriculture (Dutch Ministry of Economic Affairs)
• Role of slow release fertilizers in reducing negative impacts of nitrogen deposition on Dutch oak forests (Dutch Nature organisations)
• Developing a guidance system for the sustainable harvest of forests in view of nutrient balances (Dutch forestry organisations)

Bibliography:

2020

Amelung, A., D. Bossio, W. de Vries, I. Kögel-Knabner, J. Lehmann, R. Amundson, P. Baveye, R. Bol, C. Collins, R. Lal, J. Leifeld, B. Minasny, G. Pan, C. Rumpel, J. Sanderman, J.W. van Groenigen, B van Wesemael, M. Wander and A. Chabbi, 2020. Towards a global-scale soil climate mitigation strategy. Nat Commun 11, 5427 (2020). https://doi.org/10.1038/s41467-020-18887-7

De Vries, W. and L. Schulte-Uebbing, 2020. Required changes in nitrogen inputs and nitrogen use efficiencies to reconcile agricultural productivity with water and air quality objectives in the EU-27. Proceedings 842 of the International Fertiliser Society, 39 pp.

De Vries, W., A. de Jong, J. Kros and J. Spijker, 2020. Use of soil nutrient balances in deriving forest biomass harvesting guidelines in dependence of region, tree species and soil type. Forest Ecol and Man. 479. https://doi.org/10.1016/j.foreco.2020.118591

Etzold, S., M. Ferretti, G. J. Reinds, S. Solberg, A. Gessler, P. Waldner, M. Schaub, D. Simpson, S. Benham, K. Hansen, M. Ingerslev, M. Jonard, P-E. Karlsson, A-J. Lindroos, A. Marchetto, M. Manninger, H. Meesenburg, P. Merilä, P. Nöjd, P. Rautio, T. Sanders, W. Seidling, M. Skudnik, A, Thimonier, A. Verstraeten, L Vesterdal, M. Vejpustkova and W. de Vries, 2020. Nitrogen deposition is the most important environmental driver of growth of pure, even aged and managed European forests. Forest Ecology and Management 458, https://doi.org/10.1016/j.foreco.2019.117762

Flechard, C.R., A. Ibrom, U.M. Skiba, W. de Vries, M. van Oijen, D.R. Cameron, N.B.Dise, J.F.J. Korhonen, N. Buchmann, A. Legout, D. Simpson, M.J. Sanz, M. Aubinet, D. Loustau, L. Montagnani, J. Neirynck, I.A. Janssens, M. Pihlatie, R. Kiese, J. Siemens, A.-J. Francez, J. Augustin, A. Varlagin, J. Olejnik, R. Juszczak, M. Aurela, B.H. Chojnicki, U. Dämmgen, V. Djuricic, J. Drewer, W. Eugster, Y. Fauvel, D. Fowler, A. Frumau, A. Granier, P. Gross, Y. Hamon, C. Helfter, A. Hensen, L. Horváth, B. Kitzler, B. Kruijt, W. L. Kutsch, R. Lobo-do-Vale, A. Lohila, B. Longdoz, M.V. Marek, G. Matteucci, M. Mitosinkova, V. Moreaux, A. Neftel, J. –M. Ourcival, K. Pilegaard, G. Pita, F. Sanz, J.K. Schjoerring, M. –T. Sebastià, Y.S. Tang, H. Uggerud, M. Urbaniak, N. van Dijk, T. Vesala, S. Vidic, C. Vincke, T.Weidinger, S. Zechmeister-Boltenstern, K. Butterbach-Bahl, E. Nemitz and M.A. Sutton, 2020 Carbon/nitrogen interactions in European forests and semi-natural vegetation. Part I: Fluxes and budgets of carbon, nitrogen and greenhouse gases from ecosystem monitoring and modelling. Biogeosciences 17: 1583–1620. https://bg.copernicus.org/articles/17/1583/2020/

Flechard, C.R., M. van Oijen, D.R. Cameron, W. de Vries, A. Ibrom, N. Buchmann, N.B. Dise, I.A. Janssens, J. Neirynck, L. Montagnani, A. Varlagin, D. Loustau, A. Legout, K. Ziemblinska, M. Aubinet, M. Aurela, B.H. Chojnicki, J. Drewer, W. Eugster, A.-J. Francez, R. Juszczak, B. Kitzler, W. L. Kutsch, A. Lohila, B. Longdoz, G. Matteucci, V. Moreaux, A. Neftel, J. Olejnik, M.J. Sanz, J. Siemens, T. Vesala, C. Vincke, E. Nemitz, S. Zechmeister-Boltenstern, K. Butterbach-Bahl, U.M Skiba and M.A. Sutton, 2020. Carbon/nitrogen interactions in European forests and semi-natural vegetation. Part II: Untangling climatic, edaphic, management and nitrogen deposition effects on carbon sequestration potentials. Biogeosciences 17: 1621–1654. https://bg.copernicus.org/articles/17/1621/2020/

Ge, X., M. Schaap, R. Kranenburg, A. Segers, G.J. Reinds, J. Kros and W. de Vries, 2020. Modeling Atmospheric Ammonia using Agricultural Emissions with Improved Spatial Variability and Temporal Dynamics. Atmos. Chem. Phys. 20, 16055–16087. https://acp.copernicus.org/articles/20/16055/2020/

Hao, T., Q. Zhu, M. Zeng, J. Shen, S. Shi, X. Liu, F.S. Zhang and W. de Vries, 2020. Impacts of nitrogen fertilizer type and application rate on soil acidification rate under a wheat-maize double cropping system. Journal of Environmental Management 270. 110888 https://doi.org/10.1016/j.jenvman.2020.110888

Kanter, D.R., W. Winiwarter, B. L. Bodirsky, A.F. Bouwman, E. Boyer, S. Buckle, J.E. Compton, T. Dalgaard, W. de Vries, D. Leclere, A. Leip, C. Müller, A. Popp, N. Raghuram, S. Rao, M.A. Sutton, H. Tian, H. Westhoek, X. Zhang and M. Zurek, 2020. A framework for nitrogen futures in the shared socioeconomic pathways. Global Environmental Change 61 102029. https://doi.org/10.1016/j.gloenvcha.2019.102029

Lade, S.J, W. Steffen, W. de Vries, S.R. Carpenter, J. F. Donges, D. Gerten, H. Hoff, T. Newbold, K. Richardson and J. Rockström, 2020. Human impacts on planetary boundaries amplified by Earth system interactions. Nature Sustainability 3: 119–128 https://doi.org/10.1038/s41893-019-0454-4

Li, T., W. Zhang, H. Cao, H. Ying, Q. Zhang, S. Ren, Z. Liu, Y. Yin, W. Qin, C. Wei; Z. Cui, X. Liu, X. Ju, O. Oenema, W. de Vries, F.S. Zhang, 2020. Region-specific nitrogen management indexes for sustainable cereal production in China. Environ. Res. Commun. 2 (2020) 075002. https://doi.org/10.1088/2515-7620/aba12d

Rodrigo-Comino, J., López-Vicente, M., Kumar, V., Rodríguez-Seijo, A., Valkó, O., Rojas, C., Pourghasemi, H.R., Salvati, L., Bakr, N., Vaudour, E., Brevik, E.C., Radziemska, M., Pulido, M., Di Prima, S., Dondini, M., de Vries, W., Santos, E.S., Mendonça-Santos, M. de L., Yu, Y., Panagos, P., 2020. Soil Science Challenges in a New Era: A Transdisciplinary Overview of Relevant Topics. Air, Soil and Water Research 13: 1-1, 1178622120977491. https://doi.org/10.1177/1178622120977491

Ros, G.H., M. Luleva and W. de Vries, 202. Soil analysis is pivotal for fertilizer recommendations: Comment on “Soil based, field specific fertilizer recommendations are a pipe-dream” by A.G.T. Schut and K.E. Giller. Geoderma https:/doi.org/10.1016/j.geoderma.2020.114680

Schmitt, E. and W. de Vries, 2020. Potential benefits of using insect frass as a soil amendement on food production and for environmental impact reduction. Current Opinion in Green and Sustainable Chemistry 25: 100335. https://www.sciencedirect.com/science/article/pii/S2452223620300225

Wamelink, G.W.W, J.P. Mol-Dijkstra, G.J. Reinds, J.C. Voogd, L.T.C. Bonten, M. Posch, S.M. Hennekens and W. De Vries, 2020. Prediction of plant species occurrence as affected by nitrogen deposition and climate change on a European scale. Environmental pollution 266 https://doi.org/10.1016/j.envpol.2020.115257

Xia, N., E. Du, X. Wu, Y. Tang, Y. Wang and W. de Vries, 2020. Effects of nitrogen addition on soil methane uptake in global forest biomes. Environmental Pollution 264, 114751

Xu, D., A. Carswell, Q. Zhu, F. Zhang and W, de Vries, 2020. Modelling long-term impacts of fertilization and liming on soil acidification at Rothamsted experimental station. Science of the total Environment https://doi.org/10.1016/j.scitotenv.2019.136249.

Young, M., G.H. Ros and W. de Vries, 2020. A decision support framework assessing management impacts on crop yield, soil carbon changes and nitrogen losses to the environment. European Journal of Soil Science https://doi.org/10.1111/ejss.13024

Zhu, Q., X. Liu, T. Hao, M. Zeng, J. Shen, F. Zhang and W. de Vries, 2020. Cropland acidification increases risk of yield losses and food insecurity in China. Environmental pollution 256 https://www.sciencedirect.com/science/article/pii/S0269749119322560

2019

Clark, C.M., J. Richkus, P.W. Jones, J. Phelan, D.Burns, W. de Vries, E. Du, M. Fenn, L. Jones and S. Watmough, 2019. A synthesis of ecosystem management strategies for forests in the face of chronic nitrogen deposition. Environmental Pollution, 248: 1046-1058.

Du, E., M.E. Fenn, W. de Vries, Y.S. Ok, 2019. Atmospheric nitrogen deposition to global forests: Status, impacts and management options. Environmental Pollution 250: 1044 -1048.

Hao, T., Q. Zhu, M. Zeng, J. Shen, X. Shi, X. Liu, F.S. Zhang and W. de Vries, 2019. Quantification of the contribution of nitrogen fertilization and crop harvesting to soil acidification in a wheat-maize double cropping system. Plant and Soil, 434: 167 – 184.

Schmitz, A., T. Sanders, A. Bolte, F. Bussotti, T. Dirnböck, J. Johnson, J. Peñuelas, M. Pollastrini, A.-K. Prescher, J. Sardans, A. Verstraeten and W. de Vries, 2019. Responses of forest ecosystems in Europe to decreasing nitrogen deposition. Environmental Pollution 244: 980-994.

Wang, X., G. Daigger, W. de Vries, C. Kroeze, M. Yang, N.-Q. Ren, J. Liu and D. Butler, 2019. Impact hotspots of reduced nutrient discharge shift across the globe with population and dietary changes. Nature Communications 10, 2627. https://doi.org/10.1038/s41467-019-10445-0

Willett, W., J. Rockström, B. Loken, M. Springmann, T. Lang, S. Vermeulen, T. Garnett, D. Tilman, A. Wood, F. DeClerck, M. Jonell, M. Clark, L. Gordon, J. Fanzo, C. Hawkes, R. Zurayk, J.A. Rivera, W. de Vries, L.M. Sibanda, A. Afshin, A. Chaudhary, M. Herrero, R. Agustina, F. Branca, A. Lartey, S. Fan, B. Crona, E. Fox, V. Bignet, M. Troell, T. Lindahl, S. Singh, S.E. Cornell, S. Reddy, S. Narain, S. Nishtar and C.J.L. Murray, 2019. Food in the Anthropocene: the EAT–Lancet Commission on healthy diets from sustainable food systems. Lancet 393(10170): 447-492. https://doi.org/10.1016/S0140-6736(18)31788-4

2018

De Vries, 2018. Soil carbon 4 per mille: a good initiative but let’s manage not only the soil but also the expectations. Geoderma 309: 111–112.

Du, E. and W. de Vries, 2018. Nitrogen-induced new net primary production and carbon sequestration in global forests. Environmental Pollution, 242: 1476-1487.

Du, E., W. de Vries, S. McNulty and M. E. Fenn, 2018. Bulk deposition of base cationic nutrients in China’s forests: annual rates and spatial characteristics. Atmospheric Environment 184: 121-128.

Kros, J., N.J. Hutchings, I.T. Kristensen, I. Sillebak Kristensen, C. D, Børgesen, J.C. Voogd, T. Dalgaard and W. de Vries, 2018. A comparison of disaggregated nitrogen budgets for Danish agriculture using Europe-wide and national approaches. Science of the total Environment 643: 890–901.

Midolo, G., R. Alkemade, A.M. Schipper, A.Benítez-López, M.P. Perring and W de Vries, 2018. Impacts of nitrogen addition on plant species richness and abundance: A global meta-analysis. Global Ecology and Biogeography: https://doi.org/10.1111/geb.12856

Schulte-Uebbing, L. and W.de Vries, 2018. Global-scale impacts of nitrogen deposition on tree carbon sequestration in tropical, temperate and boreal forests: A meta-analysis. Glob Change Biol. 2018: 416-431.

Schwede, D.B., D. Simpson, J. Tan, J.S. Fu, F. Dentener, E. Du and W. de Vries, 2018. Spatial variation of modelled total, dry and wet nitrogen deposition on forests at global scale. Environmental Pollution 243: 1287–1301.

Springmann, M., M. Clark, D. Mason-D’Croz, K. Wiebe, B.L. Bodirsky, L. Lassaletta, W. de Vries, S.J Vermeulen, M. Herrero, K.M. Carlson, M. Jonell, M. Troell, F. DeClerck, L.J. Gordon, R. Zurayk, P. Scarborough, M. Rayner, B. Loken, J. Fanzo, H.CJ. Godfray, G.D. Tilman, J. Rockström and W. Willett, 2018. Changes in food management, technology and diets to stay within planetary boundaries. Nature 562: 519–525.

Zhu, Q., X. Liu, T. Hao, M. Zeng, J. Shen, F. Zhang, and W. de Vries, 2018a. Modeling soil acidification in typical Chinese cropping systems. Science of the Total Environment 613–614: 1339–1348.

Zhu, Q., W. de Vries, X. Liu, T. Hao, M. Zeng, J. Shen and F.S Zhang, 2018b. Enhanced acidification in Chinese croplands as derived from element budgets in the period 1980-2010. Science of the total Environment 618: 1497–1505.

2017

De Vries, W., M. Posch, D. Simpson and G. J. Reinds, 2017. Modelling long-term impacts of changes in climate, nitrogen deposition and ozone exposure on carbon sequestration of European forest ecosystems. Science of the total Environment 605-606: 1097–1116.

De Vries, W., E. Du, K. Butterbach Bahl, L. Schulte-Uebbing and F. Dentener, 2017. Global-scale impact of human nitrogen fixation on greenhouse gas emissions. Oxford Research Encyclopedia of Environmental Science. doi 10.1093/acrefore/9780199389414.013.13.

Dong, D., E. Du, Z. Sun, X. Zeng and W. de Vries, 2017. Non-linear direct effects of acid rain on leaf photosynthetic rate of terrestrial plants. Environmental Pollution 231: 1442 -1445.

Du, E., D. Dong, X. Zeng, Z. Sun, X. Jiang and W. de Vries, 2017. Direct effect of acid rain on leaf chlorophyll content of terrestrial plants in China. Science of the total Environment 605–606: 764-769.

Lugato, E., L. Paniagua, A. Jones, W. de Vries, A. Leip, 2017. Complementing the top soil information of the Land Use/Land Cover Area Frame Survey (LUCAS) with modelled N2O emissions. PLoS ONE 12(4): e0176111. https://doi.org/10.1371/journal.pone.0176111

Van Dobben, H. F. and de Vries, W., 2017. The contribution of nitrogen deposition to the eutrophication signal in understorey plant communities of European forests. Ecology and Evolution 7: 214–227.

Zeng, M., W. de Vries, L.T.C Bonten, Q. Zhua, T. Hao, X. Liu, F.S.and J. Shen, 2017. Model-based analysis of the long-term effects of fertilization management on cropland soil acidification. Environmental Science and Technology 51 (7): 3843–3851.

Zhang, Y., W. de Vries, X. Hao and X. Shi, 2017. Impacts of long-term nitrogen fertilization on acid buffer rates and buffer mechanisms of a slightly calcareous clay soil. Geoderma 305: 92–99.

Zimmermann, A., H. Webber, G. Zhao, F. Ewert, J. Kros, J. Wolf, W. Britz and W. de Vries, 2017. Climate change impacts on crop yields, land use and environment in response to crop sowing dates and thermal time requirements. Agricultural Systems 157: 81–92.

2016

Du, E., W. de Vries, W. Han, X. Liu, Z. Yan and Y. Jiang, 2016. Imbalanced phosphorus and nitrogen deposition in China’s forests. Atmos. Chem. Phys., 16, 8571–8579.

Heuvelink, G.B.M., J. Kros, G. J. Reinds and W. de Vries, 2016. Geostatistical prediction and simulation of European soil property maps. Geoderma Regional 7: 201-215.

Kros, J., J.P. Mol-Dijkstra, G.W.W. Wamelink, G.J. Reinds, A. van Hinsberg and W. de Vries, 2016. Modelling changes in soil acidity, nitrogen status and plant species diversity in natural ecosystems in response to changes in acid deposition and hydrology. Ecological processes 5 (1), 22.

Kroeze, C., S. Gabbert, N. Hofstra, B. Koelmans, A. Li, A. Löhr, F. Ludwig, M. Strokal, C. Verburg, L. Vermeulen, M. van Vliet, W. de Vries, M. Wang and J van Wijnen, 2016. Global Modelling of Surface Water Quality: a Multi-Pollutant Approach. Current Opinion in Environmental Sustainability 23:35–45.

Van der Salm, C., J. Kros and W. de Vries, 2016. Evaluation of different approaches to describe the sorption and desorption of phosphorus in soils on experimental data. Science of the Total Environment 571: 292–306.

Zhu, B., J. Kros, J.P. Lesschen, I.G. Staritsky and W. de Vries, 2016. Assessment of uncertainties in greenhouse gas emission profiles of livestock sectors in Africa, Latin America and Europe. Regional Environmental Change 16 (6), 1571-1582.

Zhu, Q., W. de Vries, X. Liu, M. Zeng, T Hao, E Du, F.S.and J. Shen, 2016. The contribution of atmospheric deposition and forest harvesting to forest soil acidification in China since 1980. Atmospheric Environment 146: 215-222.

2015

De Vries, W., J. Kros, M.A. Dolman, Th.V. Vellinga, H.C. de Boer, M.P.W. Sonneveld and J. Bouma, 2015. Environmental impacts of innovative dairy farming systems aiming at improved internal nutrient cycling: a multi-scale assessment. Science of the Total Environment 536: 432–442.

Du, E., W. de Vries, X. Liu, J. Fang, J. N Galloway and Y. Jiang, 2015. Spatial boundary of urban ‘acid islands’ in China. Nature Scientific Reports 5:12625 | DOI: 10.1038/srep12625.

Kros, J., M.M. Bakker, P. Reidsma, A. Kanellopoulos, S. Jamal Alam and W. de Vries, 2015. Impacts of agricultural changes in response to climate and socio economic change on nitrogen deposition in nature reserves. Landscape Ecology 30:871–885.

Leip, A., G. Billen, J. Garnier, B. Grizzetti, L. Lassaletta, S. Reis, D. Simpson, M.A. Sutton, W. de Vries, F. Weiss and H. Westhoek, 2015. Impacts of European Livestock Production: nitrogen, sulphur, phosphorus and greenhouse gas emissions, land-use, water eutrophication and biodiversity. Environ. Res. Lett. 10 (2015) doi:10.1088/1748-9326/10/11/115004.

Reidsma, P., M.B. Bakker, A. Kanellopoulos, S.J. Alam, W. Paas, J. Kros and W. de Vries, 2015. Sustainable agricultural development in a rural area in the Netherlands? Assessing impacts of climate and socio-economic change at farm and landscape level. Agricultural Systems 141:160–173.

Steffen, W., K. Richardson, J. Rockström, S.E. Cornell, I. Fetzer, E.M. Bennett, R. Biggs, S.R. Carpenter, W. de Vries, C.A. de Wit, C. Folke, D. Gerten, J. Heinke, G.M. Mace, L.M. Persson, V. Ramanathan, B. Reyers, S. Sörlin, 2015. Planetary Boundaries: Guiding human development on a changing planet. Science 347, 1259855 (2015). DOI: 10.1126/science.1259855.

Van Grinsven, J J.M., J.W. Erisman, W. de Vries, H. Westhoek, 2015. Potential of extensification of European agriculture for a more sustainable food system; the case for nitrogen and livestock. Environ. Res. Lett. 10 (2015) 025002 doi:10.1088/1748-9326/10/2/025002.

Webber, H., G. Zhao, J. Wolf, W. Britz, W. de Vries, T. Gaiser, H. Hoffmann and F. Ewert, 2015. Climate change impacts on European crop yields: do we need to consider nitrogen limitation?. Europ. J. Agronomy 71: 123–134.

Wolf, J., A. Kanellopoulos, J. Kros, H. Webber, G. Zhao, W. Britz, G. J. Reinds, F. Ewert and W. de Vries, 2015 Combined analysis of climate, technological and price changes on future arable farming systems in Europe. Agricultural Systems 140: 56–73.

2014

De Vries, W., 2014. Nutrients trigger carbon storage. Nature climate change 4: 425-426.

De Vries, W., M.H. Dobbertin, S. Solberg, H. van Dobben and M. Schaub, 2014. Impacts of acid deposition, ozone exposure and weather conditions on forest ecosystems in Europe: an overview. Plant and Soil 380 (1): 1-45.

De Vries, W, E. Du and K. Butterbach-Bahl, 2014. Short and long-term impacts of nitrogen deposition on carbon sequestration by forest ecosystems. Current Opinion in Environmental Sustainability, 9–10: 90–104.

Du, E., W. de Vries, J. N. Galloway, X. Hu, J. Fang, 2014. Changes in wet nitrogen deposition in the United States between 1985 and 2012. Environ. Res. Lett. 9 095004 doi:10.1088/1748-9326/9/9/095004.

Du, E., Y. Jiang, J. Fang and W. de Vries, 2014. Inorganic nitrogen deposition in China’s forests: status and characteristics. Atmospheric Environment: 474-482.

Glaesner, N., K. Helming and W. de Vries, 2014. Do Current European Policies Prevent Soil Threats and Support Soil Functions?. Sustainability 6, 9538-9563.

Granath, G., J. Limpens, M. Posch, S. Mücher and W. de Vries, 2014. Spatio-temporal trends of nitrogen deposition and climate effects on Sphagnum productivity in European peatlands. Environmental Pollution 187: 73-80.

2013

Baeten, L., K.Verheyen, C.Wirth, H.Bruelheide, F.Bussotti, L.Finér, B.Jaroszewicz, F.Selvi, F. Valladares, E. Allan, E. Ampoorter, H. Auge, D.Avăcăritei, L.Barbaro, I. Bărnoaiea, J. Bauhus, C. Beinhoff, R. Benavides, A. Benneter, S. Berger, F. Berthold, J. Boberg, D. Bonal, W. Brüggemann, M. Carnol, B. Castagneyrol, Y. Charbonnier, E. Chećko, D. Coomes, A. Coppi, E. Dalmaris, G. Dănilă, S.M. Dawud, W. De Vries, H. De Wandeler, M. Deconchat, T. Domisch, G. Duduman, M. Fischer, M. Fotelli, T.E. Gimeno, A. Granier, C. Grossiord, V. Guyot, L. Hantsch, S. Hattenschwiler, A. Hector, M. Hermy, V. Holland, H. Jactel, J. Koricheva, M. Lexer, S. Müller, B. Muys, D. Nguyen, L. Nichiforel, T.C.E. Paine, M. Pollastrini, K. Radoglou, K. Raulund-Rasmussen, P. Ruiz Benito, J. Stenlid, L. Vesterdal, K. von Wilpert, M.A. Zavala, M. Scherer-Lorenzen, 20213. A novel comparative research platform designed to determine the functional significance of forest tree diversity in Europe. Persp. Plant Ecol. Evol. & Syst 15: 281– 291

Cameron, D., M. van Oijen, C. Werner, K. Butterbach-Bahl, E. Haas, G.B.M. Heuvelink, R. Grote, R. Kiese, M. Kuhnert, J. Kros, A. Leip, H. I. Reuter, G.J. Reinds, M.J. Schelhaas, W. de Vries and J. Yeluripati, 2013. Environmental change impacts on the C- and N-cycle of European forests: a model comparison study. Biogeosciences 10: 1751–1773.

Danielewska, A., E. Paoletti, N. Clarke, J. Olejnik, M. Urbaniak, M. Baran, P. Siedlecki, K. Hansen, L. Lundin, W. de Vries, T. N. Mikkelsen, S. Dillen and R. Fischer, 2013. Toward integration of research and monitoring at forest sites in Europe. Forest systems 22(3): 535-545.

Danielewska, A., N. Clarke, J. Olejnik, K. Hansen, W. de Vries, L. Lundin, J.P. Tuovinen, R. Fischer, M. Urbaniak and E. Paoletti, 2013. A meta-database comparison from various European Research Networks dedicated to forest sites. iForest, doi: 10.3832/ifor0751-006.

De Vries, W. and M.J. McLaughlin, 2013. Modeling the cadmium balance in Australian agricultural systems in view of possible impacts on food quality. Science of the Total Environment 461-462: 240–257.

De Vries, W., J. Kros, C. Kroeze and S.P. Seitzinger, 2013. Assessing planetary and regional nitrogen boundaries related to food security and adverse environmental impacts. Current Opinion in Environmental Sustainability 5:392–402.

Erisman, J.W., J. Galloway, S. Seitzinger, A. Bleeker, N.B. Dise, R. Petrescu, A. M. Leach and W. de Vries, 2013. Consequences of human modification of the global nitrogen cycle. Phil. Trans R. Soc. B 368: 20130116.http://dx.doi.org/10.1098/rstb.2013.0116.

Kros, J.,T.J.A. Gies, J.C. Voogd, R. Smidt and W. de Vries, 2013. Efficiency of agricultural measures to reduce nitrogen deposition on Natura 2000 sites. Environmental Science & Policy, 32: 68 – 79.

Schulp, C.J.E., P.H. Verburg, P. J. Kuikman, G.J. Nabuurs, J.G.J. Olivier, W. de Vries, and A Veldkamp, 2013. Improving national-scale carbon stock inventories using knowledge on land use history. Environmental Management 51:709–723.

Sutton, M. A., S. Reis, S. N. Riddick, U. Dragosits, E. Nemitz, M.R. Theobald, Y. S. Tang, C. F. Braban, M. Vieno, A. J. Dore, R. F. Mitchell, S. Wanless, F. Daunt, D. Fowler,T.D. Blackall, C. Milford, C.R. Flechard, B. Loubet, R. Massad, P. Cellier, P. F. Coheur, L. Clarisse, M. van Damme, Y. Ngadi, C. Clerbaux, C. A. Skjoth, C. Geels, O. Hertel, R.J. Wickink Kruit, R.W. Pinder, J.O. Bash, J.D. Walker, D. Simpson, L. Horvath, T.H. Misselbrook, A. Bleeker, F. Dentener and W. de Vries, 2013. Toward a climate-dependent paradigm of ammonia emission & deposition. Phil. Trans. R. Soc. B 368: 20130166. http://dx.doi.org/10.1098/rstb.2013.0166.

2012

Groenenberg, J.E., J.J. Dijkstra, L.T.C. Bonten, W. de Vries and R.N.J. Comans, 2012. Evaluation of the performance and limitations of empirical partition-relations and process based multisurface models to predict trace element solubility in soils. Environmental Pollution 166: 98-107.

Hutchings, N.J., G.J. Reinds, A. Leip, M. Wattenbach, J.F. Bienkowski, T. Dalgaard, U. Dragosits, J.L. Drouet, P. Durand, O. Maury and W. de Vries, 2012. A model for simulating the timelines of field operations at a European scale for use in complex dynamic models. Biogeosciences, 9, 4487-4496.

Kros, J., G.B.M. Heuvelink, G.J. Reinds, J.P. Lesschen, V. Ioannidi and W. de Vries, 2012. Uncertainties in model predictions of nitrogen fluxes from agro-ecosystems in Europe. Biogeosciences 9: 4573-4588.

Matyssek, R., G. Wieser, C. Calfapietra, W. de Vries, P. Dizengremel, D. Ernst, Y. Jolivet, T.N. Mikkelsen, G.M.J. Mohren, D. LeThiec, J.-P. Tuovinen, A. Weatherall and E. Paoletti, 2012. Forests under Climate Change and Air Pollution: Gaps in Understanding and Future Directions for Research. Environmental Pollution 160: 57-65.

Reinds, G.J., G. M. Heuvelink, G., T. Hoogland, J. Kros and W. de Vries, 2012. Estimating nitrogen fluxes at the European scale by upscaling INTEGRATOR model outputs from selected sites. Biogeosciences 9: 4527-4536.

Sonneveld, M.P.W., J.A. de Vos, J. Kros, M. Knotters, A. Bleeker, A. Hensen, A. Frumau, A. Bleeker and W. de Vries, 2012. Assessment of N and P status at the landscape scale using environmental models and measurements. Environmental Pollution 162: 168-175.

2011

Bonten, L.T.C., J. E Groenenberg and W. de Vries, 2011. Using advanced surface complexation models for modelling soil chemistry under forests: Solling forest, Germany. Environmental Pollution 159: 2831-2839.

Clarke N., R. Fischer, W. de Vries, L. Lundin, E. Paoletti, P. Merilä, G. Matteucci, M. Mirtl, D. Simpson and T. Vesala T, 2011. Availability, accessibility, quality and comparability of monitoring data for European forests for use in air pollution and climate change science. iForest Biogeosciences and forestry 4: 162-166.

De Vries, W. and M. Posch, 2011. Modelling the impact of nitrogen deposition, climate change and nutrient limitations on tree carbon sequestration in Europe for the period 1900–2050. Environmental Pollution 159: 2289-2299.

De Vries, W., M.J. Mc Laughlin and J.E Groenenberg, 2011. Transfer functions for solid solution partitioning of cadmium for Australian soils. Environmental Pollution 159: 3583-3594.

De Vries, W., P. Cellier, J. W. Erisman and M.A. Sutton, 2011. Assessment of nitrogen fluxes to air and water from site scale to continental scale: an overview. Environmental Pollution 159: 3143-3148.

De Vries, W., A. Leip, G. J. Reinds, J. Kros, J. P. Lesschen and A.F. Bouwman, 2011. Comparison of land nitrogen budgets for European agriculture by various modeling approaches. Environmental Pollution 159: 3253–3267.

De Vries, W., J. Kros, G. J. Reinds and K. Butterbach-Bahl, 2011. Quantifying impacts of nitrogen use in European agriculture on global warming potential. Current Opinion in Environmental Sustainability 3: 291–302.

Fischer R., W. Aas, W. de Vries, N. Clarke, P. Cudlin, D. Leaver, L. Lundin, G. Matteucci, R. Matyssek, T. Mikkelsen, M. Mirtl, Y. Öztürk, D. Papale, N. Potocic, D. Simpson, J-P. Tuovinen, T. Vesala, G. Wieser, and E. Paoletti, 2011. Towards a transnational system of supersites for forest monitoring and research in Europe – an overview on present state and future recommendations. iForest Biogeosciences and forestry 4: 167-171.

Kros, J., K.F.A Frumeau, A. Hensen and W. de Vries, 2011. Integrated analysis of the effects of agricultural management on environmental quality at landscape scale. Environmental Pollution 159: 3170–3181.

Leip, A.,W. Britz, F. Weiss and W. de Vries, 2011. Farm, Land, and Soil nitrogen budgets for Agriculture in Europe calculated with CAPRI. Environmental Pollution 159: 3242–3252.

Leip, A., M. Busto, M. Corazza, P. Bergamaschi, R. Koeble, R. Dechow, S. Monni and W. de Vries, 2011. Estimation of N2O fluxes at the regional scale: data, models, challenges. Current Opinion in Environmental Sustainability 3: :328–338.

Lesschen, J.P., G.L. Velthof, W. de Vries and J. Kros, 2011. Differentiation of nitrous oxide emission factors for agricultural soils. Environmental Pollution 159: 3215–3222.

2010

Bobbink, R., K. Hicks, J. Galloway, T. Spranger, R. Alkemade, M. Ashmore, M. Bustamante, S. Cinderby, E. Davidson, F. Dentener, B. Emmett, J.W Erisman, M. Fenn, F. Gilliam, A. Nordin, L. Pardo and W. de Vries, 2010. Global Assessment of Nitrogen Deposition Effects on Terrestrial Plant Diversity: a synthesis. Ecological Applications, 20 (1): 30–59.

Brus, D.J., W. de Vries and J.J. de Gruijter, 2010. A sampling strategy for estimating annual fluxes of chemical elements from forest soils. Geoderma 159: 399–408.

De Vries, W., G.W.W. Wamelink, H. van Dobben, J. Kros, G. J. Reinds, J..P Mol-Dijkstra, S.M. Smart, C. D. Evans, E.C. Rowe, S. Belyazid, H.U. Sverdrup, A. van Hinsberg, M.Posch, J.P. Hettelingh, T. Spranger and R. Bobbink, 2010. Use of dynamic soil-vegetation models to assess impacts of nitrogen deposition on plant species composition and to estimate critical loads: an overview. Ecological Applications, 20 (1): 60–79.

De Vries, W., J. P. Lesschen, D.A. Oudendag, J. Kros, J.C. Voogd, E. Stehfest and A.F. Bouwman, 2010. Impacts of model structure and data aggregation on European wide predictions of nitrogen and greenhouse gas fluxes in response to changes in livestock, land cover and land management. Journal of Integrative Environmental Sciences, Vol. 7, No. S1: 145–157.

De Vries, W., H.J.J. Wieggers and D.J. Brus, 2010. Impacts of sampling design and estimation methods on nutrient leaching of intensively monitored forest plots in the Netherlands. Journal of Environmental Monitoring, 12: 1515 – 1523.

Groenenberg, J.E., P.F.A.M. Römkens, R.N.J. Comans, J. Luster, T. Pampura, L. Shotbolt, E.Tipping and W. de Vries, 2010. Transfer functions for solid solution partitioning of cadmium, copper, nickel, lead and zinc: Derivation of relations for free metal ion activities and validation on independent data. European Journal of Soil Science 61: 58–73.

Hensen, A., A. Bleeker, J.W. Erisman, A. Syakila, C. Kroeze, J. Kros,W. de Vries, K. Sanders. Two N-visualisation tools: game versus reality. Journal of Integrative Environmental Sciences, Vol. 7, No. S1: 289-299.

Kros, J., W. de Vries, G. J. Reinds, J. P. Lesschen and G.L. Velthof, 2010. Assessment of the impact of various mitigation options on nitrous oxide emissions caused by the agricultural sector in Europe. Journal of Integrative Environmental Sciences, Vol. 7, No. S1: 223–234.

Nol, L., G. B. M. Heuvelink, A. Veldkamp, W. de Vries and J. Kros, 2010. Uncertainty propagation analysis of an N2O emission model at the plot and landscape scale. Geoderma 159 (1-2): 9-23.

Reinds, G.J. and W. de Vries, 2010. Uncertainties in critical loads and target loads of sulphur and nitrogen for European forests: analysis and quantification. Science of the total Environment 408:1960–1970.

Van Dobben, H and W. de Vries, 2010. Relation between forest vegetation, site conditions and atmospheric deposition at regional and European scale. Environmental Pollution 158:921–933.

2009

De Vries, W., 2009. Assessment of the relative importance of nitrogen deposition and climate change on the sequestration of carbon by forests in Europe: an overview. Forest Ecology and Management 258: vii–x.

De Vries, W., S. Solberg, M. Dobbertin, H. Sterba, D. Laubhann, M. van Oijen, C. Evans, P. Gundersen, J. Kros, G.W.W. Wamelink, G.J Reinds and M.A. Sutton, 2009. The impact of nitrogen deposition on carbon sequestration by European forests and heathlands. Forest Ecology and Management 258: 1814-1823.

De Vries, W. and J.E. Groenenberg, 2009. Evaluation of approaches to calculate critical metal loads for forest soils. Environmental Pollution 157: 3422-3432.

De Vries, W., G.J. Reinds and M.F.P Bierkens, 2009. Assessment of the optimal time interval for repeated soil surveys at intensively monitored forest plots. Journal of Environmental monitoring 11: 2009-2021.

Dise, N.B., J.J. Rothwell, V. Gauci, C. van der Salm and W de Vries, 2009. Predicting nitrate leaching in European forests using two independent databases. Science of the total Environment 407 (5): 1798 – 1808.

Laubhann, D, H. Sterba, G.J. Reinds and W. de Vries, 2009. The impact of atmospheric deposition and climate on forest growth in European monitoring plots: An empirical tree growth model. Forest Ecology and Management 258: 1751-1761.

Mol-Dijkstra, J. P., G. J. Reinds, J.Kros, B. Berg and W. de Vries, 2009. Modelling soil carbon sequestration of intensively monitored in forest plots in Europe by three different approaches. Forest Ecology and Management 258:1780–1793.

Neumann, K., B. S. Elbersen, P. H. Verburg. I. Staritsky, M. Pérez Soba, W. de Vries and W. A. Rienks, 2009. Modelling the spatial distribution of livestock in Europe. Landscape ecology 24:1207–1222.

Nol, L., G. B. M. Heuvelink, W. de Vries, J. Kros, E. J. Moors and P. H. Verburg, 2009. Effect of temporal resolution on N2O emission inventories in Dutch fen meadows. Global Biogeochemical Cycles 23, GB4003, doi:10.1029/2008GB003378.

Posch, M. and W. de Vries, 2009. Dynamic Modelling of Heavy Metals – Time Scales and Target Loads. Environmental Modelling & Software 24: 86–95.

Pussinen, A., Nabuurs, G.J., R. Wieggers, G.J. Reinds, G.W.W. Wamelink, J. Kros, J. P. Mol-Dijkstra and W. de Vries, 2009. Modelling long term impacts of environmental change on mid- and high-latitude European forests and options for adaptive forest management. Forest Ecology and Management 258: 1806-1813.

Reinds, G.J., M. Posch and W. de Vries, 2009. Modelling the long-term soil response to atmospheric deposition at intensively monitored forest plots in Europe. Environmental Pollution 157:1258–1269.

Solberg, S., M. Dobbertin, G.J. Reinds, K. Andreassen, H. Lange, P. Garcia Fernandez, A. Hildingsson and W. de Vries, 2009. Analyses of the impact of changes in atmospheric deposition and climate on forest growth in European monitoring plots: A stand growth approach. Forest Ecology and Management 258: 1735-1750.

Wamelink, G.W. W., H. F. van Dobben, J. P. Mol-Dijkstra, E. P.A.G. Schouwenberg, J. Kros, W. de Vries and F Berendse, 2009. Effect of nitrogen deposition reduction on biodiversity and carbon sequestration. Forest Ecology and Management 258: 1774-1779.

Wamelink, G.W.W., R. Wieggers, G.J. Reinds, J. Kros, J. P. Mol-Dijkstra, M. van Oijen and W. de Vries, 2009. Modelling impacts of changes in carbon dioxide concentration, climate and nitrogen deposition on growth and carbon sequestration of Intensive Forest Monitoring plots in Europe. Forest Ecology and Management 258: 1794-1805.

2008

De Vries, W., S. Solberg, M. Dobbertin, H. Sterba, D. Laubhann, G.J. Reinds, G.J. Nabuurs and P. Gundersen, 2008. Ecologically implausible carbon response?. Nature 451: E1-E3.

De Vries, W., P.F.A.M. Römkens and L.T.C. Bonten, 2008. Spatially explicit integrated risk assessment of present soil concentrations of cadmium, lead, copper and zinc in the Netherlands. Water, Air and Soil Pollution 191:199–215.

Reinds, G.J., M. Posch, W. de Vries, J. Slootweg and J.-P. Hettelingh, 2008. Critical loads of sulphur and nitrogen for terrestrial ecosystems in Europe and Northern Asia influenced by different soil chemical criteria. Water, Air and Soil Pollution 193:269–287.

Sutton, M. A., D. Simpson, P.E. Levy, R. I. Smith, S. Reis, M. van Oijen and W. de Vries, 2008. Uncertainties in the relationship between atmospheric nitrogen deposition and forest carbon sequestration. Global Change Biology 14, 2057–2063.

Name:

Eric Bishop von Wettberg

Job Title:

Assistant Professor

Email:

ebishopv@uvm.edu

Phone Number:

8026569117

Links:

Google Scholar Profile

Biography:

Dr. Eric JB von Wettberg is an Assistant Professor in the Department of Plant and Soil Science at the University of Vermont with a research program focused on the consequences of genetic bottlenecks for wild relatives of crops. He received a PhD in Ecology and Evolutionary Biology from Brown University in 2007 and was a NIH National Research Service Award postdoc at the University of California at Davis from 2007-2009.  Broadly trained in evolution, genetics, ecology and agroecology, Dr. von Wettberg uses a combination of field, greenhouse common garden, and laboratory approaches.

Many crops, like the grain legumes chickpea and pigeonpea, have lost genetic variation as a result of human cultivation and selection. The lack of genetic variation reduces resilience of these crops to expected effects of climate change. Von Wettberg and his research group are using a new collection of the wild relatives of chickpea to restore genetic variation to cultivated chickpea, and to better understand the genetic basis of flowering time and drought tolerance. In addition to altering genetic variation of the crop, domestication likely had far reaching consequences on the biodiversity of communities in centers of domestication.  How changes in plants resulting from domestication altered above and belowground plant communities is little explored communities in centers of domestication, where extended periods of human agricultural activities and modification of “natural” systems have occurred.

Research Areas:

• Domestication
• Stress tolerance
• Legumes
• Chickpea
• Medicago
• Salt tolerance

Current N-related Projects:

• Impact of domestication on chickpea-mesorhizobia nitrogen fixation

Bibliography:

: von Wettberg EJ, Chang PL, Greenspan A, Carrasquila-Garcia N, Basdemir F, Moenga S, Bedada G, Dacosta-Calheiros E, Moriuchi KS, Balcha L, Mamo B, Singh V,Cordeiro MA, Vance L, Bergmann E,  Warschefsky EJ, Marques E, Dinegde KN, Sani SG, Getahun T, Yilmaz MA, Cacmak A, Rose J, Migneault A, Krieg CP, Saylak S, Temel H, Noujdina NV, Friesen ML, Siler E, Linday D, Akhmetov Z, Ozelik H, Kholova J, Can C, Caur P, Yildirim M, Sharma H, Vadez V, Tesfaye K, Woldemedhin AF, Tar’an B, Ayodogan A, Bekun B, Penmetsa RV, Berger J, Kahraman A, Nuzhdin SV, Cook DR.  Ecology and community genomics of an important crop wild relative as a prelude to agricultural innovation. Nature Communications, in press.

Krieg CP, Valls R, Vatland S, Gordnier J, Porter SS, Friesen ML, von Wettberg EJ. Nitrogen Fixation: Fixing the Gap Between Concept and Evidence Based Learning with Legume Biology.  American Biology Teacher, in press

Varshney RK, Saxena RK, Upadhyaya HD, Khan A, Yu O, Kim C, Rathore A, Seon D, Kim J, An S, Kumar V, Anauradha G, Yamini K, Zhang W, Muniswamy Z, Kim B, Penmetsa RV, von Wettberg EJ, and Datta SK. 2017. Whole genome re-sequencing of 292 pigeonpea cultivars, landraces and wild species accessions provides targets for domestication and genomic regions associated with agronomic traits for crop improvement. Nature Genetics, doi:10.1038/ng.3872

Moriuchi KS, Friesen ML, Cordeiro MA, Badri M, Vu WT, Main BA, Arraouadi S, Aouani ME, Nuzhdin SV, Cook DR, Strauss SY, von Wettberg EJ. 2016 Transgenerational plasticity for traits underlying salinity tolerance, local adaptation, and phenotypic integration in Medicago truncatula. PlosOne DOI: 10.1371/journal.pone. 015035

Friesen ML*, von Wettberg EJ*, Badri M*, Moriuchi KS, Barhoumi F, Cuellar-Ortiz S, Chang PL, Cordeiro MA, Vu WT, Arrouadi S, Djebali N, Zribi K, Badri Y, Porter SS, Aouani ME, Cook DR, Strauss SY, Nuzhdin SV. 2014 The ecological basis of salinity adaptation in Tunisian Medicago truncatula. BMC Genomics. 15: 1160.

Castro BM, Moriuchi KS, Friesen ML, Badri M, Nuzhdin SV, Strauss SY, Cook DR, von Wettberg EJ3. 2013. Parental environments and interactions with conspecifics alter salinity tolerance of offspring in the annual Medicago truncatula. Journal of Ecology 101: 1281–1287

Friesen ML, Porter SS, Stark SC, von Wettberg EJ, Sachs J, and Martinez-Romero, E. 2011. Microbially mediated functional plant traits. Annual Review of Ecology Evolution and Systematics.  42: 23-46

Holdredge C, von Wettberg EJ, Bertness MB, Silliman BR. 2010. Differential nutrient uptake abilities underlie cryptic Phragmites invasion in New England salt marshes. Oikos. 119: 1776-1784.

Name:

Jeff Volenec

Job Title:

Professor

Email:

jvolenec@purdue.edu

Links:

Google Scholar Profile

Biography:

Volenec conducts research on physiological processes that influence productivity of crops, with an emphasis on perennial species used for forage and biomass. Stress tolerance is of particular importance in these cropping systems because of the need to survive winter and to withstand complete periodic defoliation during the growing season. In addition, perennial forages and biomass species are generally relegated to lands deemed marginal for row-crop production. Thus, they often encounter less-than-ideal levels of soil nutrients, water, drainage, and other edaphic factors. By understanding mechanisms by which plants tolerate these severe stresses, Dr. Volenec hopes to be able to identify selection criteria for use by plant breeders and/or management strategies that improve productivity and long-term persistence. Examples of Volenec’s research include:

1. Starch Reserves, Growth and Persistence. Perennial plants accumulate organic reserves that provide nutrients necessary for initial shoot growth in spring and for shoot regrowth if plants are defoliated in summer. Starch accumulates in the large carrot-like taproots of perennial legume species and in rhizomes or stolons of warm-season perennial grasses. Volenec has focused much of his research on taproot physiology and starch metabolism of alfalfa – the most important forage legume grown worldwide. Volenec and his students were the first to show that high levels of taproot starch does not necessarily lead to rapid regrowth after defoliation. Plants with as little as 5% starch in their taproots regrow as fast as or faster than comparable high-starch plants after harvest. Subsequent work indicated that the activity of certain starch-degrading enzymes (a-amylases) rather than the quantity of starch, has the greatest impact on rate of shoot regeneration following defoliation. Contrary to popular belief, β-amylase appears not to participate in starch hydrolysis in alfalfa taproots, but may instead serve as a storage protein (see below). Anatomical studies also reveal control of starch breakdown is highly regulated at the cellular level. Cells located a few millimeters from each other can have vastly different rates of starch breakdown.
2. Nitrogen Reserves and Agronomic Performance. Cold soils in spring inhibit nitrogen fixation and lead to an initial N deficiency in perennial legumes like alfalfa. In addition, nitrogen fixation is markedly reduced after defoliation, these agronomic systems are rarely fertilized with N. Volenec, and co-workers have explored the role of taproot proteins in mediating regrowth and persistence of perennial legumes. These studies have shown that a large decline in taproot protein concentration occurs during initial shoot growth in the spring and after each forage harvest in summer. More importantly, Volenec and his students have discovered specific proteins (including β-amylase) that comprise as much as 50% of the taproot protein pool that are preferentially degraded to provide N to regrowing shoots. These proteins are considered vegetative storage proteins (VSP’s). Working with mutants lacking these VSP’s, Volenec and his students have shown that regrowth rate of -VSP plants is less half that of their +VSP counterparts following defoliation. Recent results have extended these findings to perennial grasses including those used for biomass like Miscanthus and shown a strong dependency of growth of these species on rhizome protein pools.
3. Fall Dormancy and Winterhardiness. Fall dormancy also has been a research topic emphasized in Volenec’s research program. It has been recognized for some time that plants with reduced shoot growth in autumn are more winter hardy than are plants that continue shoot growth late into fall. Our understanding of the biological mechanisms underlying fall dormancy is virtually nonexistent. This is despite the fact that, more than any other single feature, fall dormancy is used to predict winter survival of important crop species like alfalfa. The goal of this research is to identify and understand the nature of genes and gene products essential for winter hardiness of alfalfa. For example, Volenec and co-worked have isolated and characterized cold acclimation-responsive genes in alfalfa and showed that nondormant alfalfa lines that winterkill do not express these genes. Other work has focused on accumulation of raffinose family oligosaccharides that accumulate in taproots of winter hardy alfalfa lines, but not those that winterkill. Volenec’s work is unique in that it uses germplasms that differ in fall growth habit and winter hardiness, but which are otherwise closely related. This approach has enabled Volenec and his associates to identify key genetic and physiological differences controlling shoot growth in autumn and winter hardiness.
4. Plant Nutrition. Proper plant nutrition, especially nitrogen (N), potassium (K) and phosphorus (P) is critical for high yield and stress tolerance. Volenec and his students are evaluating the interactions of these nutrients on agronomic performance (yield, yield components), and as importantly, the underlying physiological basis of greater growth and persistence. Improved plant persistence often occurs on K-fertilized plots, while fertilization with P without addition of K fertilizer can reduce plant persistence and yield. Changes in root starches, sugars, and N pools like proteins and amino acids are informing our knowledge of how these nutrients impact crop performance. These findings will result in improved diagnostic tools (soil tests, tissue tests) that will be helpful in managing N, P and K for improved agronomic performance, while protecting the environment.
5. Sustainable Biomass Production. Renewed in interest in production of herbaceous biomass for bioenergy has resulted in new opportunities for Volenec and collaborators to study the environmental consequences and changes in ecosystem services that occur when perennial crops are used for biofuels. Studies are PU’s Water Quality Field Station (WQFS), a field laboratory comprised of forty-eight 24 m x 9 m drainage lysimeters, permit simultaneous quantification of nutrient losses from the field to surface and ground waters, and emission of greenhouse gasses like methane, carbon dioxide and nitrous oxide. WQFS treatments were modified to include perennial biomass treatments like a low-input big bluestem prairie, Miscanthus, and switchgrass compared to continuous corn with stover biomass removal; all compared to traditional corn-soybean production that currently dominates the Midwest US cornbelt. Our goal is to quantify the N, C, and water balances for candidate biofuels cropping systems knowing that these will be critical drivers of biomass production and sustainability.
6. Data and Evidence-based Agriculture. Volenec and collaborators have been at the forefront of Ag data issues for a decade. As Program Planning Officer for the American Society of Agronomy, Volenec organized in 2010 the first-ever Big Data symposium including Simon Liu, then leader of the National Agricultural Library. Working with collaborators Ron Turco and Sylvie Brouder, Volenec co-organized an invite-only meeting in 2013 called “Smart Ag” in Washington DC to bring Ag data issues to our nation’s thought leaders in Ag. Volenec later testified in Washington on data issues following the release of the memo from the Obama administration requiring open access of all data finding by the Federal Government. He also has served as the leader of the data taskforce convened by the Alliance of Crop, Soils and Environmental Science Societies, a coalition of professional societies including Crop Science Society of America, the Soil Science Society of America, and the American Society of Agronomy. He and colleagues view data as an asset and should be freely available for repurposing. They continue to work with science librarians, industry partners, the USDA, and professional societies to create workflows, tools and repositories available for data curation. Recently they have been funded to curate data in the Purdue University Research Repository for several large multi-institutional grants, and have a standing Memorandum of Agreement with the National Agricultural Library that also focuses on curation of Ag data.

Research Areas:

• Crop physiology and ecology

Current N-related Projects:

• N cycling in agro-ecosystems

Bibliography:

Volenec, J.J., and C.J. Nelson. 1983. Response of tall fescue leaf meristems to N fertilization and harvest frequency. Crop Sci. 23:720-724.

Volenec, J.J., and C.J. Nelson. 1984. Carbohydrate metabolism in leaf meristems of tall fescue. II. Relationship to leaf elongation rates modified by nitrogen fertilization. Plant Physiol. 74:595-600.

Cherney, J.H., K.D. Johnson, J.J. Volenec, and K.S. Anliker. 1988. Chemical composition of herbaceous grass and legume species grown for maximum biomass production. Biomass 17:215-238.

MacAdam, J.W., J.J. Volenec, and C.J. Nelson. 1989. Effects of nitrogen on mesophyll cell division and epidermal cell elongation in tall fescue leaf blades. Plant Physiol. 89:549-556.

Hendershot, K.L., and J.J. Volenec. 1993. Taproot nitrogen accumulation and use in overwintering alfalfa (Medicago sativa L.). J. Plant Physiol. 141:68-74.

Hendershot, K.L., and J.J. Volenec. 1993. Nitrogen pools in taproots of Medicago sativa L. after defoliation. J. Plant Physiol. 141:129-135.

Volenec, J.J., and S.M. Cunningham. 1995. Effect of applied nitrogen on seedling growth and cotyledon protein utilization of effective and ineffective nodulating alfalfa. J. Plant Nutr. 18:1519-1534.

Cunningham, S.M., and J.J. Volenec. 1996. Purification and characterization of vegetative storage proteins from alfalfa (Medicago sativa L.) taproots. J. Plant Physiol. 147:625-632.

Li, R., J.J. Volenec, B.C. Joern, and S.M. Cunningham. 1996. Seasonal changes in nonstructural carbohydrates, protein, and macronutrients in roots of alfalfa, red clover, sweetclover, and birdsfoot trefoil. Crop Sci. 36:617-623.

Volenec, J.J., A. Ourry, and B.C. Joern. 1996. A role for nitrogen reserves in forage regrowth and stress tolerance. Physiol. Plant. 97:185-193.

Avice, J-C., A. Ourry, J.J. Volenec, G. Lemaire, and J. Boucaud. 1996. Defoliation-induced changes in abundance and immuno-localization of vegetative storage proteins in taproots of Medicago sativa. Plant Physiol. Biochem. 34:561-570.

Barber, L.D., B.C. Joern, J.J. Volenec, and S.M. Cunningham. 1996. Supplemental nitrogen effects on alfalfa regrowth and nitrogen mobilization from roots. Crop Sci. 36:1217-1223.

Li, R., J.J. Volenec, B.C. Joern, and S.M. Cunningham. 1997. Potassium and nitrogen effects on carbohydrate and protein metabolism in alfalfa roots. J. Plant Nutrition 20:511-529.

Kalengamaliro, N.E., J.J. Volenec, B.C. Joern, and S.M. Cunningham. 1997. Seedling development and deposition of starch and storage proteins in alfalfa roots. Crop Sci. 37:1194-1200.

Avice, J.C., A. Ourry, G. Lemaire, J.J. Volenec, and J. Boucaud. 1997. Root protein and vegetative storage protein are key organic nutrients for alfalfa shoot regrowth. Crop Sci. 37:1187-1193.

Li, R., J.J. Volenec, B.C. Joern, and S.M. Cunningham. 1998. Effects of phosphorus nutrition on carbohydrate and protein metabolism in alfalfa roots. J. Plant Nutrition 21:459-474.

Cunningham, S.M., and J.J. Volenec. 1998. Seasonal carbohydrate and protein metabolism in roots of contrasting alfalfa (Medicago sativa L.) cultivars. J. Plant Physiol. 153:220-225.

Noquet, C., J.C. Avice, A. Ourry, J.J. Volenec, S.M. Cunningham and J. Boucaud. 2001. Effects of environmental factors and endogenous signals on N uptake, N partitioning and taproot vegetative storage protein accumulation in Medicago sativa. Aust. J. Plant Physiol. 28:279-288.

Justes, E., P. Thiebeau, J-C. Avice, G. Lemaire, J.J. Volenec, and A. Ourry. 2002. Influence of summer sowing dates, N fertilization and irrigation on autumn VSP accumulation and dynamics of spring regrowth in alfalfa (Medicago sativa L.). J. Exp. Bot. 53:111-121.

Meuriot, F., J.-C. Avice, M.-L. Decau, J.-C. Simon, P. Laine, J.J. Volenec, and A. Ourry. 2003. Accumulation of N reserves and vegetative storage protein (VSP) in taproots of non-nodulated alfalfa (Medicago sativa L.) are affected by mineral N availability. Plant Sci. 165:709-718.

Noquet, C., F. Meuriot, J.-C. Avice, A. Ourry, S.M. Cunningham, and J.J. Volenec. 2003. Short-day photoperiod induced changes in N uptake, N partitioning and accumulation of vegetative storage proteins in two Medicago sativa L. cultivars. Func. Plant Biol. 30:853-863.

Avice, J-C., F. Le-Dily, E. Goulas, C. Noquet, F. Meuriot, J.J. Volenec, S.M. Cunningham, T.G. Sors, C. Dhont, Y. Castonguay, P. Nadeau, G. Belanger, F-P. Chalifour, and A. Ourry. 2004. Vegetative storage proteins in overwintering storage organs of forage legumes: roles and regulation. Can. J. Bot. 81:1198-1212.

Meuriot, F., C. Noquet, J.C. Avice, J.J. Volenec, S.M. Cunningham, T. Sors, S. Caillot, and A. Ourry. 2004. Methyl jasmonate alters N partitioning, N reserves accumulation and induces gene expression of a 32-kDa vegetative storage protein that possess chitinase activity in Medicago sativa L. taproots. Physiol. Plant. 120:113-123.

Meuriot, F., M.-L. Decau, A. Morvan-Bertrand, M.-P. Prud’homme, F. Gastal, J.-C. Simon, J.J. Volenec, and J.-C. Avice. 2005. Contribution of initial C and N reserves in Medicago sativa recovering from defoliation: impact of cutting height and residual leaf area. Func. Plant Biol. 32:321-334.

Patton, A.J., S.M. Cunningham, J.J. Volenec, and Z.J. Reicher. 2007. Differences in freeze tolerance of zoysiagrasses. I. Role of Proteins. Crop Sci. 47:2162-2169.
Brouder, S.M. and J.J. Volenec. 2008. Impact of climate change on crop nutrient and water use efficiencies. Physiol. Plant. 133:705-724.

Berg, W.K., S.M. Cunningham, S.M. Brouder, B.C. Joern, K.D. Johnson, J.J. Volenec. 2009. Influence of phosphorus and potassium on alfalfa yield, taproot C and N pools, and transcript levels of key genes after defoliation. Crop Sci. 49:974-982.

Pembleton, K.G., S.M. Cunningham, and J.J Volenec. 2010. Effect of summer irrigation on seasonal changes in taproot reserves and the expression of winter dormancy/activity in four contrasting lucerne cultivars. Crop Pasture Sci. 61:873-884.

Dierking, R.M., D. Allen, S.M. Brouder, and J.J. Volenec. 2016. Yield, biomass composition, and N use efficiency during establishment of four Miscanthus × giganteus genotypes as influenced by N management. Biomass Bioenergy 91:98-107.

Brouder, S.M., and J.J. Volenec. 2017. Future climate change and plant macro-nutrient use efficiency. pp. 357-379. In Plant macro-nutrient use efficiency: Molecular and genomic perspectives. Hossain, M.A., Kamiya, T., Burrit, D.J., Phan Tran, L.-S., Fujiwara, T. (Eds.). Elsevier. ISBN: 9780128113080

Dierking R.M., D.J. Allen, S.M. Cunningham, S.M. Brouder, and J.J. Volenec. 2017. Nitrogen dynamics in above and belowground tissues of two Miscanthus × giganteus genotypes under various N fertilization rates. Front. Plant Sci. DOI: 10.3389/fpls.2017.01618.

Name:

U. Vishnunath

Job Title:

Junior Research Fellow

Email:

vishnu591993@gmail.com

Phone Number:

9585731892

Biography:

 

Research Areas:

• Symbiotic association involving in N2 fixing process
• Root Nodule species involving Nitrogen fixation process
• Methods to convert sterile chemical contaminated soil into organic nutrient rich soil by using Nitrogen fixing plants, trees species.

Current N-related Projects:

• “Genetic improvement of Casuarina for growth and pulp yield through intra and interspecific hybridization” under the guidance of Dr.A.Nicodemus sir, Scientist – F and Head of GTB Division, IFGTB.

Bibliography: